ProMutor

ProMutor is platform for generating advantageous point mutants based on explicit modelling of evolutionary history.

Here, the effect of suggested mutations is evaluated by predicting their outcomes based on explicit modelling of the evolutionary history of natural sequences. The resulting multiple-sequence-alignment-based conservation scores \(\Delta \Delta E\) quantify how likely each of the 19 substitutions are in terms of what has been observed in the evolutionary record, with \(\Delta \Delta E \approx 0\) corresponding to very conservative substitutions (have minor effect on protein structure) and the lower \(\Delta \Delta E\) is, the more likely those mutations disrupt protein's structure. Note, that one should compare scores between mutants with different numbers of introduced mutations. In addition, scores should not be interpreted as a quantitative measure of protein's properties (e.g. stability). They are used to rank mutants and select the most advantageous ones (those with the highest evolutionary score among others). For more information regarding the parameter choice please consult our documentation as well as our tutorial on how to use ProMutor.

Sequence type

DNA

Protein

Insert your sequence

Choose file to upload

or paste a sequence

At what positions do you want to generate mutations?

At what positions do you want to insert mutation(s)?
Put \(0\) for screening mode (scoring all possible point mutations)

At what position do you ALWAYS want to insert a mutation?
(Select from the above mentioned positions)

What is the maximum number of mutations to include per mutant?

Should be only 1 integral number or empty

Do you expect a preserved or disrupted structure?

Preserved

Disrupted

What substitution matrix do you want to use?

Default: Blosum62

Which sequence database do you want to use?

Note that in general it is better to use a bigger database (UniClust) to get more reliable predictions
Default: Uniclust

Nota bene: The calculations might take several minutes, please do not quit the page in order to receive your results below.